Engineering novel amyloid biofilm based material for capture and degradation of micro-plastics

Study Code / Acronym
Award Number
Status / Stage
31 January 2022 -
30 January 2023
Duration (calculated)
00 years 11 months
Funding Amount
Funder/Grant study page
Contracted Centre
University of Kent
Principal Investigator
Dr Wei-Feng Xue
PI Contact
WHO Catergories
Understanding Underlying Disease
Disease Type
Dementia (Unspecified)

CPEC Review Info
Reference ID745
ResearcherReside Team


Study Code / Acronym21ENGBIO
Award NumberBB/W011530/1
Status / StageActive
Start Date20220131
End Date20230130
Duration (calculated) 00 years 11 months
Funder/Grant study pageBBSRC UKRI
Contracted CentreUniversity of Kent
Funding Amount£100,527.00


To produce a novel amyloid mash material decorated with PET degrading enzymes, we will first produce chimera protein monomers using the pET/BL21 protein expression system. The chimera proteins will consist of the amyloid forming part of the yeast protein Sup35 (called Sup35NM) linked to PETase, MHETase or BHETase. These three enzymes will provide Sup35NM amyloid fibrils with enzymatic activities that catalyse a series of reactions that breaks down PET into ethylene glycol and terephthalic acid. Secondly, we will make constructs containing the three catalytic chimera proteins for expression and extracellular export into the media as monomers, where they can assemble in situ into large fibril networks, using the Curli-dependant amyloid generator (C-DAG) system. We will also assemble the chimera proteins into amyloid fibril networks decorated with PET degrading enzymes in vitro, which we will confirm by kinetics and AFM imaging experiments. Finally, we will assess the structure and the PET degrading activity of the fibril mesh material formed in vitro and in situ. Thus, this projeect will produce a novel biomaterial which is a robust yet malleable, non-toxic substance that could act as microplastics capturing and degrading filters.